Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics.
At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial
pathogens in various specimens.
MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.
Bluetongue (BT) is an infectious, non -contagious, vector-borne viral disease that affects wild and domestic ruminants such as sheep, goats, cattle, buffaloes, deer, most species of African antelope and various other vertebrate hosts. It is transmitted by midges, small insects, of the genus Culicoides. In sheep,Bluetongue virus (BTV) causes an acute disease with high morbidity and mortality. Infection of cattle with BTV does not usually result in clinical signs, with the exception of BTV 8 infection in Europe. Cattle are particularly significant in the epidemiology of the disease due to the prolonged viraemia in the absence of clinical disease. Clinical signs of BT include fever, hyperaemia and congestion, swelling of the face and tongue and cyanosis of the tongue. However in mild cases of the disease, a transitory hyperaemia and slight ocular and nasal discharge may be observed.
Method
Real-Time RT-PCR
Sample Type
Accredited :
EDTA Blood.
Alternatives :
Culture, Tissue.
Transport Condition
Samples should be transported at 4°C and delivered within 24h of collection.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Bornavirus is a non-enveloped negative strand ribonucleic acid (RNA) virus. Because it uniquely replicates in the cell nucleus, it has been classified in its own family, Bornaviridae. In 2008, ABV was identified as the causative agent of proventricular dilatation disease (PDD) in psittacines, but the primary reservoir for ABV appears to be waterfowl.
The epidemiology of avian bornavirus infections is poorly characterized, including the natural reservoir(s) of infection and routes of virus transmission. The incubation period in experimentally infected birds ranges from 20 to 200 days after inoculation. Bornavirus RNA can be detected in the feces and cloacal and crop contents of naturally infected birds
Samples should be transported at 4°C. Tissue and swabs must be sent in RNA preservative media.
It is not required to add RNA preservative media to blood.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Bovine virus diarrhea or bovine viral diarrhea (BVD)or bovine viral diarrhoea (UK English) is a disease of cattle which reduces productivity and increases death loss. It is caused by a Pestivirus from the family Flaviviridae. Classical swine fever (CSF) is also caused by a pestivirus. CSF and sometimes BVD are notifiable diseases and eradication programs are administered in many countries worldwide.
The molecular biology of pestiviruses shares many similarities and peculiarities with the human hepaciviruses. Pestiviruses have the ability to establish persistent infection during pregnancy. Persistent infection with pestiviruses often goes unnoticed; for BVDV frequently nonhomologous RNA recombination events lead to the appearance of genetically distinct viruses that are lethal to the host.
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Brucellosis is a contagious disease of livestock caused by several species of the genus Brucella, mainly Brucella abortus, B. melitensis, and B. suis. Infection with Brucella in cattle is usually caused by B. abortus, less frequently by B. melitensis, and occasionally by B. suis. Brucella melitensis is the main causative agent of infection with Brucella in sheep and goats. Infection with Brucella in pigs is due to B. suis biovars 1-3, but the disease caused by biovar 2 differs in its host range, its limited geographical distribution, and its pathogenicity. Clinically, infection with Brucella in animals is characterized by one or more of the following signs: abortion, retained placenta, orchitis, epididymitis and, rarely, arthritis, with excretion of the organisms in uterine discharges and in milk. Diagnosis depends on the isolation of Brucella from abortion material, udder secretions or from tissues removed at post-mortem.
Pathogens Tested
APB-023 :Brucella abortus (This Assay includes the detection of Brucella species and Brucella abortus)
APB-024 :Brucella melitensis (This Assay includes the detection of Brucella species and Brucella melitensis)
APB-025 :Brucella Species (This Assay includes the detection of Brucella species only. This Assay can detect many Brucella species including B.abortus, B.melitensis, B.suis and B. canis)
Samples should be transported at 4°C. Milk must be frozen after collection.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Burkholderia mallei is a gram negative bipolar aerobic bacterium belonging to the genus Burkholderia. It causes a contagious and fatal disease in horses, donkeys, and mules which is called Glanders. According to the location of the initial infection, the disease presents itself in four different forms; nasal, pulmonary, cutaneous and asymptomatic carrier. The nasal and pulmonary forms tend to be more acute while the cutaneous form is a chronic process. Inflammatory nodules and ulcers develop in the nasal passages and give rise to a sticky yellow discharge. Stellate scarring follows upon healing of the ulcers. The formation of nodular abscesses in the lungs is accompanied by progressive debility, coughing and may also be accompanied by diarrhoea. In the cutaneous form (farcy), the lymph vessels are enlarged; nodular abscesses form along their course, which then ulcerate and discharge yellow pus. Nodules are regularly found in the liver and spleen, leading to wasting and death.Control of glanders requires early detection and diagnostic testing of suspected clinical cases, screening of apparently normal equids, and elimination of positive cases.
Burkholderia pseudomallei infects animals and causes the disease melioidosis. B. pseudomallei is an opportunistic pathogen and affects many animal species; infection generally results from grazing on contaminated soil or drinking contaminated water. Infected animals can excrete the organism in saliva, pus, urine, and feces. Severe disease occurs in sheep and goats but cattle, dogs, cats, horses, buffalo, rodents, camels, nonhuman primates, some species of birds, and tropical fish, also get infected. The incubation period for animals is variable ranging from a few days to many years. Some abscesses are carried asymptotically. The signs of melioidosis in animals vary depending on species, but generally include depression, fever, weight loss, respiratory signs (heavy breathing, sneezing), lameness and swelling of the joints, and potentially death. Any animals showing signs of illness should be promptly isolated.
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics.
At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial
pathogens in various specimens.
MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.
Bluetongue (BT) is an infectious, non -contagious, vector-borne viral disease that affects wild and domestic ruminants such as sheep, goats, cattle, buffaloes, deer, most species of African antelope and various other vertebrate hosts. It is transmitted by midges, small insects, of the genus Culicoides. In sheep,Bluetongue virus (BTV) causes an acute disease with high morbidity and mortality. Infection of cattle with BTV does not usually result in clinical signs, with the exception of BTV 8 infection in Europe. Cattle are particularly significant in the epidemiology of the disease due to the prolonged viraemia in the absence of clinical disease. Clinical signs of BT include fever, hyperaemia and congestion, swelling of the face and tongue and cyanosis of the tongue. However in mild cases of the disease, a transitory hyperaemia and slight ocular and nasal discharge may be observed.
Method
Real-Time RT-PCR
Sample Type
Accredited :
EDTA Blood.
Alternatives :
Culture, Tissue.
Transport Condition
Samples should be transported at 4°C and delivered within 24h of collection.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Bornavirus is a non-enveloped negative strand ribonucleic acid (RNA) virus. Because it uniquely replicates in the cell nucleus, it has been classified in its own family, Bornaviridae. In 2008, ABV was identified as the causative agent of proventricular dilatation disease (PDD) in psittacines, but the primary reservoir for ABV appears to be waterfowl.
The epidemiology of avian bornavirus infections is poorly characterized, including the natural reservoir(s) of infection and routes of virus transmission. The incubation period in experimentally infected birds ranges from 20 to 200 days after inoculation. Bornavirus RNA can be detected in the feces and cloacal and crop contents of naturally infected birds
Samples should be transported at 4°C. Tissue and swabs must be sent in RNA preservative media.
It is not required to add RNA preservative media to blood.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Bovine virus diarrhea or bovine viral diarrhea (BVD)or bovine viral diarrhoea (UK English) is a disease of cattle which reduces productivity and increases death loss. It is caused by a Pestivirus from the family Flaviviridae. Classical swine fever (CSF) is also caused by a pestivirus. CSF and sometimes BVD are notifiable diseases and eradication programs are administered in many countries worldwide.
The molecular biology of pestiviruses shares many similarities and peculiarities with the human hepaciviruses. Pestiviruses have the ability to establish persistent infection during pregnancy. Persistent infection with pestiviruses often goes unnoticed; for BVDV frequently nonhomologous RNA recombination events lead to the appearance of genetically distinct viruses that are lethal to the host.
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Brucellosis is a contagious disease of livestock caused by several species of the genus Brucella, mainly Brucella abortus, B. melitensis, and B. suis. Infection with Brucella in cattle is usually caused by B. abortus, less frequently by B. melitensis, and occasionally by B. suis. Brucella melitensis is the main causative agent of infection with Brucella in sheep and goats. Infection with Brucella in pigs is due to B. suis biovars 1-3, but the disease caused by biovar 2 differs in its host range, its limited geographical distribution, and its pathogenicity. Clinically, infection with Brucella in animals is characterized by one or more of the following signs: abortion, retained placenta, orchitis, epididymitis and, rarely, arthritis, with excretion of the organisms in uterine discharges and in milk. Diagnosis depends on the isolation of Brucella from abortion material, udder secretions or from tissues removed at post-mortem.
Pathogens Tested
APB-023 :Brucella abortus (This Assay includes the detection of Brucella species and Brucella abortus)
APB-024 :Brucella melitensis (This Assay includes the detection of Brucella species and Brucella melitensis)
APB-025 :Brucella Species (This Assay includes the detection of Brucella species only. This Assay can detect many Brucella species including B.abortus, B.melitensis, B.suis and B. canis)
Samples should be transported at 4°C. Milk must be frozen after collection.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Burkholderia mallei is a gram negative bipolar aerobic bacterium belonging to the genus Burkholderia. It causes a contagious and fatal disease in horses, donkeys, and mules which is called Glanders. According to the location of the initial infection, the disease presents itself in four different forms; nasal, pulmonary, cutaneous and asymptomatic carrier. The nasal and pulmonary forms tend to be more acute while the cutaneous form is a chronic process. Inflammatory nodules and ulcers develop in the nasal passages and give rise to a sticky yellow discharge. Stellate scarring follows upon healing of the ulcers. The formation of nodular abscesses in the lungs is accompanied by progressive debility, coughing and may also be accompanied by diarrhoea. In the cutaneous form (farcy), the lymph vessels are enlarged; nodular abscesses form along their course, which then ulcerate and discharge yellow pus. Nodules are regularly found in the liver and spleen, leading to wasting and death.Control of glanders requires early detection and diagnostic testing of suspected clinical cases, screening of apparently normal equids, and elimination of positive cases.
Burkholderia pseudomallei infects animals and causes the disease melioidosis. B. pseudomallei is an opportunistic pathogen and affects many animal species; infection generally results from grazing on contaminated soil or drinking contaminated water. Infected animals can excrete the organism in saliva, pus, urine, and feces. Severe disease occurs in sheep and goats but cattle, dogs, cats, horses, buffalo, rodents, camels, nonhuman primates, some species of birds, and tropical fish, also get infected. The incubation period for animals is variable ranging from a few days to many years. Some abscesses are carried asymptotically. The signs of melioidosis in animals vary depending on species, but generally include depression, fever, weight loss, respiratory signs (heavy breathing, sneezing), lameness and swelling of the joints, and potentially death. Any animals showing signs of illness should be promptly isolated.
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
